桃金娘造林技术研究

对桃金娘移植造林技术进行了试验研究。移栽成活率达95．6％。试验结果表明，桃金娘不仅是优良的乡土绿化树种，而且还是耐瘠薄干旱的荒山绿化美化和水土保持树种。     

山区林农创业项目的遴选

江西省林权制度改革取得了显著的成效，森林资源增值，农民增收，大大鼓舞林农务林的积极性，林农创业的浪潮已在全省勃然兴起，但创业项目的遴选必须精心运筹，仔细策划，否则一举失误。追悔莫及。     

不同剂量25羟基维生素D3对断奶前犊牛血液生化指标、抗氧化能力和免疫功能的影响

为研究不同剂量25羟基维生素D₃[25(OH)VD₃]对断奶前犊牛血液水平、抗氧化能力和免疫功能的影响,选取18 头健康状况良好的新生荷斯坦犊牛,随机分成3 组,每组6 头进行为期56 天的饲养试验。分为对照组[不添加25(OH)VD₃]、低剂量组[25(OH)VD₃添加量6 000 IU/头·天]和高剂量组[25(OH)VD₃添加量12 000 IU/头·天]。结果表明,与对照组相比,随着25(OH)VD₃添加剂量增加,血浆生长激素、胰岛素样生长因子-1、过氧化氢酶、超氧化物歧化酶、总抗氧化能力以及免疫球蛋白G(IgG)含量显著增加(P<0.05),而丙二醛含量随剂量增加而存在降低趋势(P=0.079)。与对照组和低剂量组相比,高剂量组血浆免疫球蛋白A(IgA)含量有增加趋势(P=0.059)。综上所述,断奶前犊牛饲喂高剂量25(OH)VD₃有利于促进体内生长激素和胰岛素样生长因子-1分泌,可提高犊牛抗氧化能力,增强免疫功能。     

Effects of noninvasive delayed limb ischemic preconditioning on prognosis of myocardial infarction

AIM: To study the effects of noninvasive delayed limb ischemia preconditioning (NDLIP) on animal cardiac function, myocardial morphology and myocardial apoptosis after myocardial infarction (MI). METHODS: Healthy SD male rats[n=45, weighing (250±10) g] were randomly divided into 3 groups:MI group:the animal model of MI was established by surgical ligation of left anterior descending artery (LAD) after 2 weeks; NDLIP group:after the success of the MI animal model, NDLIP was carried out every other day until the 4th, 6th and 8th weeks; sham group:as the negative control group, the animals were taken heart LAD threading but no ligation. All rats were fed conventionally. At the end of the 4th, 6th and 8th weeks, all rats were made ventricular intubation, and then the hemodynamic parameters were recorded. The blood samples were withdrawn from the abdominal aorta and the serum was separated via centrifugation. The serum contents of Bcl-2 and Bax were measured by ELISA. Left ventricular anterior wall was homogenized. The mitochondrial respiratory chain complexes Ⅰ, Ⅱ, Ⅲ and Ⅳ in the myocardial tissues were detected by ELISA. RESULTS: At the end of the 4th, 6th and 8th weeks, compared with MI group, left ventricular systolic pressure in NDLIP group was significantly increased, while left ventricular end-diastolic pressure in NDLIP group was significantly decreased (both P<0.05). Mitochondrial respiratory chain complexesⅠ, Ⅱ, Ⅲ and Ⅳ in NDLIP group were significantly increased (P<0.05). The serum level of Bcl-2 in NDLIP group was significantly increased and Bax level was reduced remarkably (both P<0.01). CONCLUSION: NDLIP improves the hemodynamic indexes, promotes the mitochondrial respiratory function and inhibits cell apoptosis, thus improving the prognosis of MI.     

氮钾配施对设施番茄土壤微生物群落及土壤养分和盐分的影响

为了探明不同氮钾肥用量对番茄根际土壤细菌群落及土壤养分和盐分的影响,以番茄为研究对象,设置不同氮肥和钾肥用量,通过土培盆栽模拟试验,利用高通量测序技术测定根际土壤微生物及土壤养分的变化。结果表明:1)施肥使主要的优势菌群发生明显改变,过量施肥显著降低根际土壤的细菌数量,根际土壤中肠杆菌科(Enterobacteriaceae)细菌的相对丰度明显下降,但鞘脂单胞菌科(Sphingomonadaceae)和黄单胞菌科(Xanthomonadaceae)相对丰度略有增加,细菌种群分布趋于单一化。2)过量施肥导致的土壤电导率(EC)增加、C/N下降,是土壤根际细菌群落发生改变的主要原因。3)过量施肥显著降低番茄株高、茎粗,但叶片叶绿素SPAD相对含量却呈现增加趋势,叶片光合作用受到抑制,从而产量明显下降。研究表明,施肥过量是导致设施土壤次生盐渍化的主要因素,次生盐渍化导致土壤中细菌群落发生改变,植株生长受到抑制,从而产量下降。     

PCR检测猪输血传播病毒Ⅰ型方法的建立及其初步应用

参照GenBank已公布的猪源输血传播病毒Ⅰ型（Torque Teno Virus genogroupⅠ,TTVⅠ）全基因序列,设计并合成一对特异性引物,PCR扩增片段为194bp,测序结果与已公布的猪TTVⅠ型序列同源性为92.6%。用所建立的方法检测猪圆环病毒Ⅱ型、猪细小病毒、猪伪狂犬病病毒、猪多杀性巴氏杆菌、猪链...     

新疆吐鲁番地区‘无核白’葡萄水分和矿质元素周年吸收规律研究

为探究新疆吐鲁番地区‘无核白’葡萄水分和矿质元素的周年吸收规律,以4年生‘无核白’葡萄为试材,采用密闭式营养液循环供给系统栽培,避免了水分和矿质元素的流失,并设置低、中、高浓度(0.5、1.0和1.5倍标准配方浓度)的Hoagland营养液处理。在葡萄植株的生长发育过程中持续测定营养生长(新梢长度、新梢粗度、叶片面积)和生殖生长(果粒横径、果粒纵径、单果质量、可溶性固形物、可滴定酸)指标,检测和计算了不同发育阶段葡萄植株对水分和矿质元素的吸收量,并测定葡萄叶片和果实中矿质元素的含量变化。结果表明,中浓度营养液栽培的‘无核白’葡萄长势中庸,果实品质最佳,作为吐鲁番地区葡萄水分和矿质营养吸收量的参考依据,其生理需水量为每年4 149.5m~3·hm~(-2),矿质元素每公顷的吸收量分别为N 227.9 kg,P 22.9 kg,K 325.9 kg,Ca 341.8 kg,Mg 117.8 kg,Fe 1.9 kg,Mn 460.6 g,Cu 182.3 g,Zn 198.1 g,B 728.3 g。花后47~75 d,‘无核白’葡萄植株的水分和矿质元素吸收速率最大。叶片和果实中的矿质元素含量总体呈高浓度营养液处理中浓度营养液处理低浓度营养液处理的趋势。     

山东省家蚕种质资源研究进展

山东省蚕业研究所保存的家蚕品种资源,从刚建所的30多个增加到现在的110多个,利用这些品种资源已育成了10对实用品种。为更好地利用种质资源,近年来进行了家蚕抗高温、抗氟及淀粉酶活性等方面的研究。     

煤矿井下电气设备防爆壳体结构研究

当前煤炭开采技术,对煤炭电气设备的安全性能提出更高要求.文章依据现阶段应用电气设备的实际情况,研究其防爆安全性能和电气设备防爆壳体,分析了煤矿井下电气设备防爆壳体结构,以便能够提高设备安全性.     

Research Progress on Technique of Frozen Embryo Transfer in Sheep

The paper introduced the research progress on the technique of frozen embryo transfer in sheep,illustrated selection of donors and receptors,superovulation,synchronization of estrus,embryo cryopreservation and embryo transplantation. Frozen embryo transfer in sheep is another breakthrough in the high-quality sheep raising,and this technique in China is in its infancy recommendation stage,but it will be comprehensively popularized in the future.